RB1 (phospho S795) monoclonal antibody, clone 28B5
产品名称： RB1 (phospho S795) monoclonal antibody, clone 28B5
英文名称： RB1 (phospho S795) monoclonal antibody, clone 28B5
产品编号： MAB6764
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic phosphopeptide of RB1.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S795 of human RB1.
- Host:
- Mouse
- Reactivity:
- Human
- Specificity:
- This antibody specific to human RB1 phosphorylated at Ser795. It can detect endogenous levels of RB1 phosphorylated at Ser795 in most cell lines.
- Form:
- Liquid
- Isotype:
- IgG2a, kappa
- Storage Buffer:
- In antiserum (0.05% sodium azide)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cancer cell cycles.
Sherr CJ.Science. 1996 Dec 6;274(5293):1672-7.
- 2.
- E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.
Nevins JR.Science. 1992 Oct 16;258(5081):424-9.
- 3.
- The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations.
Hu QJ, Dyson N, Harlow E.EMBO J. 1990 Apr;9(4):1147-55.
- Applications
- Western Blot
- Specificity of the RB1 (phospho S795) monoclonal antibody, clone 28B5 (Cat # MAB6764) as demonstrated by western blotting.
Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with monoclonal antibody 28B5.
Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo.
- Entrez GeneID:
- 5925
- Gene Name:
- RB1
- Gene Alias:
- OSRC,RB,p105-Rb,pRb,pp110
- Gene Description:
- retinoblastoma 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
- Other Designations:
- OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein
- Related Disease
- Adenocarcinoma
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Carcinoma, Hepatocellular
- Carcinoma, Squamous Cell
- Chromosomal Instability
- Chromosome Aberrations
- Chromosome Deletion
- Colorectal Neoplasms
- Cystadenocarcinoma, Serous
- Disease Progression
- Esophageal Neoplasms
- Genetic Predisposition to Disease
- Head and Neck Neoplasms
- Liver Neoplasms
- Lung Neoplasms
- Lymphoma, Mantle-Cell
- Neoplasm Invasiveness