FGFR2 polyclonal antibody
产品名称： FGFR2 polyclonal antibody
英文名称： FGFR2 polyclonal antibody
产品编号： PAB3041
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of FGFR2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human FGFR2.
- Host:
- Rabbit
- Reactivity:
- Human, Monkey, Mouse, Rat
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Conditional activation of fibroblast growth factor receptor (FGFR) 1, but not FGFR2, in prostate cancer cells leads to increased osteopontin induction, extracellular signal-regulated kinase activation, and in vivo proliferation.
Freeman KW, Gangula RD, Welm BE, Ozen M, Foster BA, Rosen JM, Ittmann M, Greenberg NM, Spencer DM.Cancer Res. 2003 Oct 1;63(19):6237-43.
- 2.
- Evidence for selective advantage of pathogenic FGFR2 mutations in the male germ line.
Goriely A, McVean GA, Rojmyr M, Ingemarsson B, Wilkie AO.Science. 2003 Aug 1;301(5633):643-6.
- 3.
- P63 alpha mutations lead to aberrant splicing of keratinocyte growth factor receptor in the Hay-Wells syndrome.
Fomenkov A, Huang YP, Topaloglu O, Brechman A, Osada M, Fomenkova T, Yuriditsky E, Trink B, Sidransky D, Ratovitski E.J Biol Chem. 2003 Jun 27;278(26):23906-14. Epub 2003 Apr 10.
- Applications
- Western Blot (Cell lysate)
- The FGFR2 polyclonal antibody (Cat # PAB3041) is used in Western blot to detect FGFR2 in Jurkat cell lysate.
- Western Blot (Transfected lysate)
- Western blot analysis of FGFR2 polyclonal antibody (Cat # PAB3041) in 293 cell line lysates transiently transfected with the FGFR2 gene (2 ug/lane). hFGFR2-R22 (arrow) was detected using the purified polyclonal antibody (1 : 30 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with FGFR2 polyclonal antibody (Cat # PAB3041) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Immunofluorescence
- FGFR2/isolectinB4 (A) and FGFR1/isolectinB4 (B) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in C). F: FGFR2 (D) and FGFR1 (E) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in F).
- Entrez GeneID:
- 2263
- Protein Accession#:
- NP_075261;P21802
- Gene Name:
- FGFR2
- Gene Alias:
- BEK,BFR-1,CD332,CEK3,CFD1,ECT1,FLJ98662,JWS,K-SAM,KGFR,TK14,TK25
- Gene Description:
- fibroblast growth factor receptor 2
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq
- Other Designations:
- BEK fibroblast growth factor receptor,FGF receptor,OTTHUMP00000020621,OTTHUMP00000020629,bacteria-expressed kinase,hydroxyaryl-protein kinase,keratinocyte growth factor receptor,protein tyrosine kinase, receptor like 14,soluble FGFR4 variant 4
- Interactome
- Related Disease
- Acrocephalosyndactylia
- Adenocarcinoma
- Adenocarcinoma, Mucinous
- Alzheimer Disease
- Alzheimer disease
- Birth Weight
- Breast cancer
- Breast Neoplasms
- Bronchial Hyperreactivity
- Carcinoma, Basal Cell
- Carcinoma, Endometrioid
- Carcinoma, Squamous Cell
- Cardiovascular Diseases
- Cleft Lip
- Cleft Palate
- Craniofacial Dysostosis
- Craniosynostoses
- Cystadenocarcinoma, Serous
- Depressive Disorder, Major