NLK (C-term) Peptide
产品名称： NLK (C-term) Peptide
英文名称： NLK (C-term) Peptide
产品编号： P2554
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of NLK.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NLK.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells.
Yasuda J, Tsuchiya A, Yamada T, Sakamoto M, Sekiya T, Hirohashi S.Biochem Biophys Res Commun. 2003 Aug 22;308(2):227-33.
- 2.
- Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling.
Ishitani T, Ninomiya-Tsuji J, Matsumoto K.Mol Cell Biol. 2003 Feb;23(4):1379-89.
- 3.
- The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling.
Ishitani T, Kishida S, Hyodo-Miura J, Ueno N, Yasuda J, Waterman M, Shibuya H, Moon RT, Ninomiya-Tsuji J, Matsumoto K.Mol Cell Biol. 2003 Jan;23(1):131-9.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of NLK polyclonal antibody (Cat # PAB2944) in A-375 cell lysate. NLK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NLK polyclonal antibody (Cat # PAB2944) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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