FITC标记的RNA结合蛋白LIN28B抗体
产品名称： FITC标记的RNA结合蛋白LIN28B抗体
英文名称： Anti-Lin-28B/FITC
产品编号： HZ-6446R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-Lin-28B/FITC Conjugated antibody
FITC标记的RNA结合蛋白LIN28B抗体
| 产品编号 | bs-6446R-FITC |
| 英文名称 | Anti-Lin-28B/FITC |
| 中文名称 | FITC标记的RNA结合蛋白LIN28B抗体 |
| 别 名 | Lin28B; CSDD 2; CSDD2; FLJ16517; Lin 28 homolog B (C. elegans); Lin 28.2; Lin-28B; LIN28B; LN28B_HUMAN; Protein lin-28 homolog B. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Lin-28B |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Function: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Subunit: Interacts with ZCCHC11/TUT4. Subcellular Location: Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively. Tissue Specificity: High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Similarity: Belongs to the lin-28 family. Contains 2 CCHC-type zinc fingers. Contains 1 CSD (cold-shock) domain. Database links: Entrez Gene: 389421 Human Entrez Gene: 380669 Mouse Omim: 611044 Human SwissProt: Q6ZN17 Human SwissProt: Q45KJ6 Mouse Unigene: 23616 Human Unigene: 440328 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
通过特异性结合前体LIT-7（前LIT-7），miRNA前体作为microRNA（miRNA）生物合成的抑制剂。通过结合前LIT-7和招募ZCCHC11/Tut4尿苷酰基转移酶，从而导致前-LIT-7的末端尿苷化。尿苷酰化前LIT-7 miRNAs不能被Dicer处理并经历降解。具体地识别前-LIT-7的末端环中的5’-GGA-3’基序。此外，还识别和结合了包含5’-GGAG-3基序的非前-7前miRNAs在末端环中，导致它们的末端尿苷化和随后的降解。介导MYC介导的LIT-7抑制。异构体1，当过表达，刺激乳腺癌细胞株MCF-7的生长。异构体2对细胞生长没有影响。