FITC标记的有丝分裂阻滞缺陷2样蛋白2抗体
产品名称： FITC标记的有丝分裂阻滞缺陷2样蛋白2抗体
英文名称： Anti-Mad2L2/FITC
产品编号： HZ-7840R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-Mad2L2/FITC Conjugated antibody
FITC标记的有丝分裂阻滞缺陷2样蛋白2抗体
| 英文名称 | Anti-Mad2L2/FITC |
| 中文名称 | FITC标记的有丝分裂阻滞缺陷2样蛋白2抗体 |
| 别 名 | Homolog of REV7 S cerevisiae; HREV7; MAD2 homolog; MAD2 like 2; MAD2 mitotic arrest deficient like 2; MAD2B; Mitotic Arrest Deficient 2 L2; Mitotic arrest deficient homolog like 2; Mitotic arrest deficient like 2 (yeast); Mitotic arrest deficient yeast homolog; Mitotic spindle assembly checkpoint protein MAD2B; REV 7; REV7; Weakly similar to Mitotic MAD2 protein (S cerevisiae). |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 23kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mad2L2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. Function: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. Subunit: Homooligomer (Probable). Interacts with REV1. Interacts with ADAM9. Interacts with CHAMP1. Interacts with REV3L. Interacts with FZR1 (in complex with the anaphase promoting complex APC). Interacts with CDC20; PubMed:11459825 could not detect the interaction. Interacts with RAN. Interacts with ELK1; the interaction is direct and recruits MAD2L2 to ELK1-specific promoters. May interact with the JNK kinases MAPK8 and/or MAPK9 to stimulate ELK1 phosphorylation and transcriptional activity upon DNA damage. Interacts with TCF7L2; prevents its binding to promoters and negatively modulates its transcriptional activity. Interacts with YY1AP1. Interacts with S. flexneri protein ipaB; prevents the interaction of MAD2L2 with FZR1 and CDC20 resulting in an activation of the anaphase-promoting complex APC and a cell cycle arrest. Interacts with PRCC; the interaction is direct. Interacts with POGZ. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Tissue Specificity: Ubiquitously expressed. Similarity: Contains 1 HORMA domain. Database links: UniProtKB/Swiss-Prot: Q9UI95.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
适配器蛋白能够与不同的蛋白质相互作用，并参与不同的生物学过程。介导易出错的DNA聚合酶Zeta催化亚基ReV3L与插入体聚合酶ReV1之间的相互作用，从而介导第二聚合酶转换在跨损伤DNA合成中的作用。跨损伤DNA合成释放复制聚合酶的复制阻断，停滞在DNA损伤的存在下。还可以通过调节JNK介导的转录激活因子ELK1的磷酸化和激活来调节细胞对DNA损伤的另一方面。抑制FZR1和可能的CDC20介导的促后期复合物APC的活化，从而调节通过细胞周期的进展。调节TCF7L2介导的基因转录，可能在上皮间质转分化中起作用。