HIST1H3B (phospho S10) polyclonal antibody
产品名称： HIST1H3B (phospho S10) polyclonal antibody
英文名称： HIST1H3B (phospho S10) polyclonal antibody
产品编号： PAB0482
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of HIST1H3B.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S10 of human HIST1H3B.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Regulation of HIV-1 gene expression by histone acetylation and factor recruitment at the LTR promoter.
Lusic M, Marcello A, Cereseto A, Giacca M.EMBO J. 2003 Dec 15;22(24):6550-61.
- 2.
- Enhancement of the p300 HAT activity by HIV-1 Tat on chromatin DNA.
Deng L, Wang D, de la Fuente C, Wang L, Li H, Lee CG, Donnelly R, Wade JD, Lambert P, Kashanchi F.Virology. 2001 Oct 25;289(2):312-26.
- 3.
- Acetylation of HIV-1 Tat by CBP/P300 increases transcription of integrated HIV-1 genome and enhances binding to core histones.
Deng L, de la Fuente C, Fu P, Wang L, Donnelly R, Wade JD, Lambert P, Li H, Lee CG, Kashanchi F.Virology. 2000 Nov 25;277(2):278-95.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of HIST1H3B (phospho S10) polyclonal antibody (Cat # PAB0482) in HepG2 cell lysate (35 ug/lane).Phospho-HIST1H3B-pS10 (arrow) was detected using the purified polyclonal antibody (1 : 64 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with HIST1H3B (phospho S10) polyclonal antibody (Cat # PAB0482) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 8358
- Protein Accession#:
- NP_003528;Q71DJ3
- Gene Name:
- HIST1H3B
- Gene Alias:
- H3/l,H3FL
- Gene Description:
- histone cluster 1, H3b
- Omim ID:
- 602819
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
- Other Designations:
- H3 histone family, member L,OTTHUMP00000016132,histone 1, H3b
- Gene Pathway