Synonyms

EPO; Erythropoietin; EP; MVCD2; Epoetin

Source

HEK293 Cells

Molecular Weight

The recombinant mouse Epo predicts a molecular mass of 18.6 kDa.

AA Sequence

NP_031968.1 ((Met1-Arg192)

Biological Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The ED50 for this effect is typically 2-10 ng/mL.

Purity

> 95% by SDS-PAGE.

Endotoxin

<1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.