PSD4 (C-term) Peptide
产品名称： PSD4 (C-term) Peptide
英文名称： PSD4 (C-term) Peptide
产品编号： P0866
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PSMD4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PSMD4.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a.
Walters KJ, Kleijnen MF, Goh AM, Wagner G, Howley PM.Biochemistry. 2002 Feb 12;41(6):1767-77.
- 2.
- Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes.
Kawahara H, Kasahara M, Nishiyama A, Ohsumi K, Goto T, Kishimoto T, Saeki Y, Yokosawa H, Shimbara N, Murata S, Chiba T, Suzuki K, Tanaka K.EMBO J. 2000 Aug 1;19(15):4144-53.
- 3.
- Hybrid proteasomes. Induction by interferon-gamma and contribution to ATP-dependent proteolysis.
Tanahashi N, Murakami Y, Minami Y, Shimbara N, Hendil KB, Tanaka K.J Biol Chem. 2000 May 12;275(19):14336-45.
- Applications
- Western Blot (Cell lysate)
- The PSMD4 polyclonal antibody (Cat # PAB1741) is used in Western blot to detect PSMD4 in Jurkat cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PSMD4 polyclonal antibody (Cat # PAB1741) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 5710
- Gene Name:
- PSMD4
- Gene Alias:
- AF,AF-1,ASF,MCB1,Rpn10,S5A,pUB-R5
- Gene Description:
- proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
- Omim ID:
- 601648
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq
- Other Designations:
- 26S protease subunit S5a,26S proteasome non-ATPase regulatory subunit 4,OTTHUMP00000014286,OTTHUMP00000059963,S5a/antisecretory factor protein,angiocidin,antisecretory factor 1,multiubiquitin chain binding protein,proteasome 26S non-ATPase subunit 4
- Gene Pathway